LOCUS       sequence-103474-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human ubc13, E2 for synthesis k63 linked ubiquitin chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Wiener R, Zhang X, Wang T, Wolberger C
  TITLE     The mechanism of OTUB1-mediated inhibition of ubiquitination.
  JOURNAL   Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
  PUBMED    22367539
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-22- 22"; volume: "483"; issue: "7391"; 
            pages: "618-22"
COMMENT     Sequence Label: hUbc13
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(795..812)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             827..847
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cagccggatc tcagtggtgg tggtggtggt gctcgattaa cccttttcga actgcgggtg
       61 gctccaggcc tcgattaaat attattcatg gcatatagcc tagtccatgc tctagctgtt
      121 tctatggctt gggcttcgtt ggtcttccac tgctccgcta catcatttgc taatggatca
      181 tctggattgg gagcacttaa caaggcctgg atcgatagca gaactgtgcg gatctgcagt
      241 gctggggacc acttatcttt caaaatatct aaacatattc ttcccaactt gtctacatta
      301 ggatgataaa ttttggtcat gaaacgtact ttaggggctg ccattgggta ttcttctgga
      361 aggaatagtt caagtttaaa agtccctccc tcaaaggggg aatcctgagg gccagcaatg
      421 accacatgaa aataacgggc gttgctctca tctggttcgg ctttgatgcc aggaactggt
      481 tctgccagca aacgctgggt ttccttgatg atcctgcggg gcagcccggc cattcctccc
      541 gtctgctgtt ggaacacatc aattgtatct tcatcctcca tttccaactg tgcaggtgtg
      601 tctgtttcat tgattggttg cccgtcaaat cggaatctga tctgcctcat tgacaatccc
      661 tgtcgttcac aataggcttt cattagttta ctaagtggtg tatgcctctt aatcttaaac
      721 tgcaccacag aaccatcctg ccccgccacc ttcaaattaa tatgatcgtt gttctcagtc
      781 ttgactcctg acggatgatg atgatgatga tggcccatat gtatatctcc ttcttaaagt
      841 taaacaa
//
