LOCUS       sequence-103475-         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 L3-L2
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(215..544)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      229..364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     529..549
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtatctaga
      121 tgcatgctcg agcggccgcc agtgtgatgg atatctgcag aattccagca cactggcggc
      181 cgttactagt ggatccgagc tcggtaccaa gctttttgca aaagcctagg cctccaaaaa
      241 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
      301 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
      361 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
      421 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
      481 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
      541 acacattcca cagcaacttt attatacaaa g
//
