LOCUS       sequence-103482-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (72P) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Vise PD, Baral B, Latos AJ, Daughdrill GW
  TITLE     NMR chemical shift and relaxation measurements provide evidence for 
            the coupled folding and binding of the p53 transactivation domain.
  JOURNAL   Nucleic Acids Res. 2005 Apr 11;33(7):2061-77. Print 2005.
  PUBMED    15824059
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-04-11"; volume: "33(7)"; pages: "2061-77. 
            Print 2005"
COMMENT     Sequence Label: pET28-p53 (1-73) (72P)
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             325..342
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(395..413)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      409..456
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(580..599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgatgg aggagccgca gtcagatcct
      121 agcgtcgagc cccctctgag tcaggaaaca ttttcagacc tatggaaact acttcctgaa
      181 aacaacgttc tgtccccctt gccgtcccaa gcaatggatg atttgatgct gtccccggac
      241 gatattgaac aatggttcac tgaagaccca ggtccagatg aagctcccag aatgccagag
      301 gctgctcccc ccgtgtgact cgagcaccac caccaccacc actgagatcc ggctgctaac
      361 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      421 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      481 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      781 tcttt
//
