LOCUS       sequence-103483-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (72P) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Vise PD, Baral B, Latos AJ, Daughdrill GW
  TITLE     NMR chemical shift and relaxation measurements provide evidence for 
            the coupled folding and binding of the p53 transactivation domain.
  JOURNAL   Nucleic Acids Res. 2005 Apr 11;33(7):2061-77. Print 2005.
  PUBMED    15824059
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-04-11"; volume: "33(7)"; pages: "2061-77. 
            Print 2005"
COMMENT     Sequence Label: pET28-p53 (1-73) (72P)
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(263..280)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(290..307)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             326..348
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    363..387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(387..406)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(388..406)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(490..509)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagtcacac ggggggagca gcctctggca
       61 ttctgggagc ttcatctgga cctgggtctt cagtgaacca ttgttcaata tcgtccgggg
      121 acagcatcaa atcatccatt gcttgggacg gcaaggggga cagaacgttg ttttcaggaa
      181 gtagtttcca taggtctgaa aatgtttcct gactcagagg gggctcgacg ctaggatctg
      241 actgcggctc ctccatcata tggctgccgc gcggcaccag gccgctgctg tgatgatgat
      301 gatgatggct gctgcccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      361 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
      421 agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      481 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      541 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      601 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      661 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccg
//
