LOCUS       sequence-103489-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV tdTomato R4-R3
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..243
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     418..441
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(548..564)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(549..565)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    578..701
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
       61 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      121 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
      181 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
      241 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
      301 gcgagatcca ccaggccctg aagctgaagg acggcggccg ctacctggtg gagttcaaga
      361 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
      421 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
      481 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaagaa ttcgatatca
      541 agcttatcga taccgtcgac ctcgagcctc tagaggacaa ctttgtataa taaagttgaa
      601 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      661 actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta cttagatggt
      721 attagtgacc tgtactgca
//
