LOCUS       sequence-10349-e        4932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: ph2p
FEATURES             Location/Qualifiers
     source          1..4932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(373..375,376..378,379..1092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(373..375,376..378,379..1089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1182..1201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1663..1931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    1973..1997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2977..3001)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3021..3593
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      3764..4352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4253..4272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4438..4485
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4477..4495
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagt aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     1141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1321 aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     1681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     1741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     1801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     1861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     1921 cgttacagat ccaagctgtg accggcgcct acgtaagtga tagcttgatc aaacaagttt
     1981 gtacaaaaaa gcaggctgcg gccgcaatca tgggagcagc cctcaccatc ctggtggatc
     2041 tgattgaagg actggctgag gtcagcacat tgactggact gagcgccgag gccatcctga
     2101 gcggcgaggc actggccgca ctggacgggg agatcaccgc cctcaccctc gaaggcgtga
     2161 tgtccagcga aaccgcattg gccaccatgg gcatcagcga agaagtctac ggcttcgtgt
     2221 ccaccgtccc cgtcttcgtg aacaggaccg ccggcgccat ctggctcatg caaacagtcc
     2281 agggcgcaag caccatcagt ttgggaatcc agagatatct gcataacgag gaagtgccca
     2341 ccgtgaaccg caacatggcc ctcatcccat ggcgcgaccc cgcactgctg gacatctact
     2401 ttccgggcgt caaccaattc gcccacgcct tgaacgtggt gcacgactgg gggcacggcc
     2461 tgctgcactc cgtcggacgc tacgtctggc agatggtcgt ccaagagacc cagcatcgcc
     2521 tcgagggcgc cgtccgcgag ttgaccgtcc gccaaaccca cacatttctg gacgggctgg
     2581 cccgcctgct ggagaataca agatgggtcg tcagcaacgc cccacaaagc gcaatcgacg
     2641 ccatcaaccg cggcgcaagc agcgtctcaa gcggatactc gagcttgtct gattactacc
     2701 gccagctggg actgaaccca ccccaacgga gagcactgtt caacaggatc gagggatcaa
     2761 tgggcaacgg aggcccaaca cccgccgccc acatccaaga cgaaagcggc gaagtcatca
     2821 agttctacca agcacccggc ggcgcacatc agcgcgtgac ccccgattgg atgttgcccc
     2881 tgatcctggg cctctatggc gacattaccc ccacctgggc caccgtgatc gaagaggacg
     2941 ggccacagaa gaagaaacgc aggttgtgat gctagcaccc agctttcttg tacaaagtgg
     3001 ttcgatctag aatggctagc attatcccta atacctgcca ccccactctt aatcagtggt
     3061 ggaagaacgg tctcagaact gtttgtttca attggccatt taagtttagt agtaaaagac
     3121 tggttaatga taacaatgca tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac
     3181 cactttggtt ttcttttttg cgtgtggcag ttttaagtta ttagttttta aaatcagtac
     3241 tttttaatgg aaacaacttg accaaaaatt tgtcacagaa ttttgagacc cattaaaaaa
     3301 gttaaatgag aaacctgtgt gttcctttgg tcaacaccga gacatttagg tgaaagacat
     3361 ctaattctgg ttttacgaat ctggaaactt cttgaaaatg taattcttga gttaacactt
     3421 ctgggtggag aatagggttg ttttcccccc acataattgg aaggggaagg aatatcattt
     3481 aaagctatgg gagggtttct ttgattacaa cactggagag aaatgcagca tgttgctgat
     3541 tgcctgtcac taaaacaggc caaaaactga gtccttgggt tgcatagaaa gcttcatgtt
     3601 gctaaaccaa tgttaagtga atctttggaa acaaaatgtt tccaaattac tgggatgtgc
     3661 atgttgaaac gtgggttaat taactagcca tgaccaaaat cccttaacgt gagttttcgt
     3721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4141 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4381 tcctggcctt ttgctggcct tttgctcaca tgttcttaat taaatttttc aaaagtagtt
     4441 gacaattaat catcggcata gtatatcggc atagtataat acgactcact ataggagggc
     4501 catcatggcc aagttgacca gtgctgtccc agtgctcaca gccagggatg tggctggagc
     4561 tgttgagttc tggactgaca ggttggggtt ctccagagat tttgtggagg atgactttgc
     4621 aggtgtggtc agagatgatg tcaccctgtt catctcagca gtccaggacc aggtggtgcc
     4681 tgacaacacc ctggcttggg tgtgggtgag aggactggat gagctgtatg ctgagtggag
     4741 tgaggtggtc tccaccaact tcagggatgc cagtggccct gccatgacag agattggaga
     4801 gcagccctgg gggagagagt ttgccctgag agacccagca ggcaactgtg tgcactttgt
     4861 ggcagaggag caggactgag gataagaatt gtaacaaaaa accccgcccc ggcggggttt
     4921 tttgttaatt aa
//
