LOCUS       sequence-103490-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (K24N) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Zhan YA, Wu H, Powell AT, Daughdrill GW, Ytreberg FM
  TITLE     Impact of the K24N mutation on the transactivation domain of p53 and
            its binding to murine double-minute clone 2.
  JOURNAL   Proteins. 2013 Oct;81(10):1738-47. doi: 10.1002/prot.24310. Epub 
            2013 Jul 22.
  PUBMED    23609977
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/prot.24310"; journalName: "Proteins"; date: "2013-10"; 
            volume: "81"; issue: "10"; pages: "1738-47"
COMMENT     Sequence Label: pET28-p53 (1-73) (K24N)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     290..309
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     393..412
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        393..411
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    412..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             451..473
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             492..509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             519..536
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             771..788
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtnggtnntt ncaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
       61 gttttgcgcc attcgtatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca
      121 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
      181 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
      241 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
      301 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
      361 cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
      421 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca
      481 tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc ggcagccata
      541 tgatggagga gccgcagtca gatcctagcg tcgagccccc tctgagtcag gaaacatttt
      601 cagacctatg gaatctactt cctgaaaaca acgttctgtc ccccttgccg tcccaagcaa
      661 tggatgattt gatgctgtcc ccggacgata ttgaacaatg gttcactgaa gacccaggtc
      721 cagatgaagc tcccagaatg ccagaggctg ctccccgcgt gtgactcgag caccaccacc
      781 accaccactg agatccggct
//
