LOCUS       sequence-103491-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (K24N) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Zhan YA, Wu H, Powell AT, Daughdrill GW, Ytreberg FM
  TITLE     Impact of the K24N mutation on the transactivation domain of p53 and
            its binding to murine double-minute clone 2.
  JOURNAL   Proteins. 2013 Oct;81(10):1738-47. doi: 10.1002/prot.24310. Epub 
            2013 Jul 22.
  PUBMED    23609977
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/prot.24310"; journalName: "Proteins"; date: "2013-10"; 
            volume: "81"; issue: "10"; pages: "1738-47"
COMMENT     Sequence Label: pET28-p53 (1-73) (K24N)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     267..286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     370..389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        370..388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    389..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             428..450
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             469..486
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             496..513
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             748..765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgtatggtgt
       61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
      121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      421 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
      481 catcacagca gcggcctggt gccgcgcggc agccatatga tggaggagcc gcagtcagat
      541 cctagcgtcg agccccctct gagtcaggaa acattttcag acctatggaa tctacttcct
      601 gaaaacaacg ttctgtcccc cttgccgtcc caagcaatgg atgatttgat gctgtccccg
      661 gacgatattg aacaatggtt cactgaagac ccaggtccag atgaagctcc cagaatgcca
      721 gaggctgctc cccgcgtgtg actcgagcac caccaccacc accactgaga tccggct
//
