LOCUS       sequence-103498-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of cyclin B1 non-degradable L45A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly 
            checkpoint responsive to kinetochore attachment.
  JOURNAL   Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347.
  PUBMED    21926987
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-09-18- 18"; volume: "13"; issue: "10"; 
            pages: "1234-43"
COMMENT     Sequence Label: pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    186..210
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tgatcacaag tttgtacaaa aaagcaggct ccgagatggc gctccgagtc accaggaact
      241 cgaaaattaa tgctgaaaat aaggcgaaga tcaacatggc aggcgcaaag cgcgttccta
      301 cggcccctgc tgcaacctcc aagcccggac tgaggccaag aacagctgct ggggacattg
      361 gtaacaaagt cagtgaacaa ctgcaggcca aaatgcctat gaagaaggaa gcaaaacctt
      421 cagctactgg aaaagtcatc gataaaaaac taccaaaacc tcttgaaaag gtacctatgc
      481 tggtgccagt gccagtgtct gagccagtgc cagagccaga acctgagcca gaacctgagc
      541 ctgttaaaga agaaaaactt tcgcctgagc ctattttggt tgatactgcc tctccaagcc
      601 caatggaaac atctggatgt gcccctgcag aagaagacct gtgtcaggct ttctctgatg
      661 taattcttgc agtaaatgat gtggatgcag aagatggagc tgatccaaac ctttgtagtg
      721 aatatgtgaa agatatttat gcttatctga gacaacttga ggaagagcaa gcagtcagac
      781 caaaatacct actgggtcgg gaagtcactg gaaacatgag agccatccta attgactggc
      841 tagtacaggt tcaaatgaaa ttcaggttgt tgcaggagac catgtacatg actgtctcca
      901 ttattgatcg gttcatgcag aataattgtg tgcccaagaa gatgctgcag ctggttggtg
      961 tcactgccat gtttattgca agcaaatatg aagaaatgta ccctccagaa attggtgact
     1021 ttgcttttgt gactgacaac a
//
