LOCUS       sequence-103501-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and sgRNA scaffold module for gRNA expression. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..78
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        122..370
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     122..142
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     293..312
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt atantgccaa
       61 ctttgtatac aaaagttgct atcgatcacg agactagcct cgagcggccg cccccttcac
      121 cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      181 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      241 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      301 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      361 acgaaacacc ggtgtgcagg tgagtgatcc agatctttac gccccgccct gccactcatc
      421 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      481 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      541 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      601 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
      661 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtag
//
