LOCUS       sequence-103503-         437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L5-L2
FEATURES             Location/Qualifiers
     source          1..437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..95
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     16..35
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(325..424)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
       61 gatttgggaa tcttataagt tctgtatgag accacagatc tcggcgttgt gcatgtcggg
      121 cgactgctcc atgatcttgc gccgctcgat ctgcgaccac accataaagg cgttcatggg
      181 ccgcttgatg tggccactgg gcgtcttgca ccagctgggg tcgtccgcct tgccgcccgt
      241 cgacgcggtg gagccaggcg tcggggagga cgcgatgccc agctccaggc cggcgcccga
      301 gtccgagctc ttttttgaat tcgtacccag ctttcttgta caaagttggc attataagaa
      361 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      421 tttgccatcc agctgca
//
