LOCUS       sequence-10351-e        4463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Pastrana DV, Tolstov YL, Becker JC, Moore PS, Chang Y, Buck CB
  TITLE     Quantitation of human seroresponsiveness to Merkel cell 
            polyomavirus.
  JOURNAL   PLoS Pathog. 2009 Sep . 5(9):e1000578.
  PUBMED    19750217
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Sep . 5(9):e1000578."
COMMENT     Sequence Label: phGluc
FEATURES             Location/Qualifiers
     source          1..4463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(373..375,376..378,379..1092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(373..375,376..378,379..1089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1182..1201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1663..1931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1963..1972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1969..2526
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     polyA_signal    2552..3124
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      3295..3883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3784..3803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3969..4016
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4008..4026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagt aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     1141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1321 aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     1681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     1741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     1801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     1861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     1921 cgttacagat ccaagctgtg accggcgcct acctcggatc cagccaccat gggagtcaaa
     1981 gttctgtttg ccctgatctg catcgctgtg gccgaggcca agcccaccga gaacaacgaa
     2041 gacttcaaca tcgtggccgt ggccagcaac ttcgcgacca cggatctcga tgctgaccgc
     2101 gggaagttgc ccggcaagaa gctgccgctg gaggtgctca aagagatgga agccaatgcc
     2161 cggaaagctg gctgcaccag gggctgtctg atctgcctgt cccacatcaa gtgcacgccc
     2221 aagatgaaga agttcatccc aggacgctgc cacacctacg aaggcgacaa agagtccgca
     2281 cagggcggca taggcgaggc gatcgtcgac attcctgaga ttcctgggtt caaggacttg
     2341 gagcccatgg agcagttcat cgcacaggtc gatctgtgtg tggactgcac aactggctgc
     2401 ctcaaagggc ttgccaacgt gcagtgttct gacctgctca agaagtggct gccgcaacgc
     2461 tgtgcgacct ttgccagcaa gatccagggc caggtggaca agatcaaggg ggccggtggt
     2521 gactaagcgg ccgctcgagc atgcatctag cattatccct aatacctgcc accccactct
     2581 taatcagtgg tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag
     2641 tagtaaaaga ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat
     2701 gttttgtgga ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt
     2761 aaaatcagta ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac
     2821 ccattaaaaa agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag
     2881 gtgaaagaca tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg
     2941 agttaacact tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag
     3001 gaatatcatt taaagctatg ggagggtttc tttgattaca acactggaga gaaatgcagc
     3061 atgttgctga ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa
     3121 agcttcatgt tgctaaacca atgttaagtg aatctttgga aacaaaatgt ttccaaatta
     3181 ctgggatgtg catgttgaaa cgtgggttaa ttaactagcc atgaccaaaa tcccttaacg
     3241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3421 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttcttaa ttaaattttt
     3961 caaaagtagt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac
     4021 tataggaggg ccatcatggc caagttgacc agtgctgtcc cagtgctcac agccagggat
     4081 gtggctggag ctgttgagtt ctggactgac aggttggggt tctccagaga ttttgtggag
     4141 gatgactttg caggtgtggt cagagatgat gtcaccctgt tcatctcagc agtccaggac
     4201 caggtggtgc ctgacaacac cctggcttgg gtgtgggtga gaggactgga tgagctgtat
     4261 gctgagtgga gtgaggtggt ctccaccaac ttcagggatg ccagtggccc tgccatgaca
     4321 gagattggag agcagccctg ggggagagag tttgccctga gagacccagc aggcaactgt
     4381 gtgcactttg tggcagagga gcaggactga ggataagaat tgtaacaaaa aaccccgccc
     4441 cggcggggtt ttttgttaat taa
//
