LOCUS       sequence-103510-       11125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein 
            Scaffold GBD:SH3:PDZ (1:1:4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p168-759
FEATURES             Location/Qualifiers
     source          1..11125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             219..230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1287..1331
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1384..1431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1484..1508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1523..1545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2624..2668
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2721..2768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2802..2820
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2821..2845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2860..2882
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4072..4116
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4169..4216
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4250..4268
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4269..4293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4308..4330
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6060..6104
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      6157..6204
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6248..6703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6545..6566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(6818..7609,7610..7678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7441..7460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7679..7771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7852..8440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8341..8360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8594..8611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8626..8768
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8694..8713)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8854..8876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8870..9061)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9633..10715)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10677..10696
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10716..10793)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10999..11018
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(11109..11125,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
      121 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
      181 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
      241 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
      301 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
      361 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
      421 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
      481 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
      541 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
      601 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
      661 aagtggtgat caacttctat ccgaaatgtc cgcagccgga tctgaccctg ggtctgaaac
      721 gtcataccga tccgggtaca attaccctgc tgctgcaaga tcaggtgggt ggtctgcagg
      781 caacccgtga tggtggcaaa acctggatta ccgttcagcc ggttgaaggt gcatttgttg
      841 ttaatctggg tgatcatggc cattatctga gcaatggtcg ctttaaaaac gcagatcatc
      901 aggcagttgt taatagcaat tgtagccgtc tgagcattgc aacctttcag aatccggcac
      961 cggaagcaac cgtttatccg ctgaaaattc gtgaaggtga aaaaccgatt ctggaagaac
     1021 cgattacctt tgccgatatg tataaacgca aaatgagcaa agatatcgag ctggccaaac
     1081 tgaaaaaact ggcgaaagaa aaaaaactgc tgcaagacca gcaggatatc gaaaaagcaa
     1141 aactggaaat caaaagcacc gatgaaatct tcgccctggt tggtgcactg atgcatgtta
     1201 tgcagaaacg tagccgtgca attcatagca gtgatgaagg tgaagatcaa gccggtgatg
     1261 aagatgagga ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
     1321 acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa
     1381 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctaggatcg
     1441 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     1501 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgggtct
     1561 gggtgcagaa agcggtagcg tttgtgttac cggtgcaagc ggttttgttg gtagctggct
     1621 ggttatgcgt ctgctggaac atggttatac cgttcgtgca accgtgcgtg atccggcaaa
     1681 tctgaaaaaa gttcgtcatc tgctggaact gccgcaggca gcaacccgtc tgaccctgtg
     1741 gaaagcagat ctggatgttg aaggtagctt tgatgaagcc attaaaggtt gtaccggtgt
     1801 ttttcatgtt gcaaccccga tggattttga aagcgaagat ccggaaaacg aagttattaa
     1861 accgaccatt aacggcatgc tggatattat gaaagcatgc ctgaaagcaa aaaccgttcg
     1921 tcgtctggtt tttaccagca gtgccggtgc agttgcaatt gaagaacatc cgaaagaagt
     1981 gtacagcgaa aataactggt cagatgttgt gttttgccgc aaagttaaaa tgaccggctg
     2041 gatgtatttt gtgagcaaaa ccctggcaga acaggcagca tggaaatttg caaaagaaaa
     2101 caacatcgac ttcatcacca ttattccgac cctggttatt ggtccgtttc tggcaccgag
     2161 catgcctccg agcctgatta gcggtctgag tccgctgacc ggtaatgaag cacattatgg
     2221 tattatcaaa cagtgccagt atgtgcatct ggatgatctg tgtcagagcc atatttttct
     2281 gtatgaacat gcaaaagccg agggtcgtta tatttgtagc agccatgatg caaccattca
     2341 cgatattgca aaactgctga acgagaaata cccgaaatac aacgttccga aaaaattcaa
     2401 aggcatcgaa gaaaacctga ccaacattca ctttagcagc aaaaaactga aagagatggg
     2461 cttcgaattt aaacacagcc tggaagatat gtttacaggt gccgttgatg catgtcgtga
     2521 aaaaggtctg ctgccgctgc cgcaagaaga agaaaccgaa aaacgtcgtg caggtcctcc
     2581 tcctgcactg cctccgaaac gtcgtcgtta actcgagtct ggtaaagaaa ccgctgctgc
     2641 gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta acctaaactg
     2701 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     2761 gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg
     2821 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
     2881 gatatacata tgaccgttag cggtgcaatt ccgagcatga ccaaaaatcg taccctggtt
     2941 gttggtggca ccggttttat tggtcagttt attaccaaag caagcctggg ttttggttat
     3001 ccgacctttc tgctggttcg tccgggtccg gttagcccga gcaaagcagt tattatcaaa
     3061 acctttcagg ataaaggtgc caaagtgatt tatggcgtga tcaacgataa agaatgcatg
     3121 gaaaaaattc tgaaagagta cgagatcgac gttgttatta gcctggtggg tggtgcacgt
     3181 ctgctggatc agctgaccct gctggaagca attaaaagcg ttaaaaccat caaacgtttt
     3241 ctgccgagcg aatttggcca tgatgttgat cgtaccgatc cggttgaacc gggtctgacc
     3301 atgtataaag aaaaacgtct ggtgcgtcgt gccgttgaag aatatggtat tccgtttacc
     3361 aatatctgct gcaatagcat tgcaagctgg ccgtattatg ataattgtca tccgagccag
     3421 gttccgcctc cgatggatca gtttcagatt tatggtgatg gtaacaccaa agcctatttc
     3481 attgatggca acgatatcgg caaatttacc atgaaaacca tcgatgatat tcgcaccctg
     3541 aacaaaaatg ttcattttcg tccgagcagc aactgctaca gcattaatga actggcaagc
     3601 ctgtgggaga aaaaaatcgg tcgtacactg cctcgtttta ccgttaccgc agataaactg
     3661 ctggcacatg cagcagaaaa cattattccg gaaagcattg ttagcagctt tacccacgat
     3721 atctttatta acggttgcca ggtgaacttt agcatcgatg aacatagtga tgtggaaatc
     3781 gatacactgt atccggatga aaaatttcgt agcctggatg attgctatga agattttgtt
     3841 ccgatggtgc acgataaaat tcatgcaggt aaaagcggtg aaatcaaaat caaagatggt
     3901 aaaccgctgg ttcagaccgg caccattgaa gaaattaaca aagacattaa aaccctggtg
     3961 gaaacccagc cgaatgaaga gatcaaaaaa gatatgaaag cactggttga agccgttccg
     4021 attagcgcaa tgggtggtgt taaagaaagc ctggtttaac tcgagtctgg taaagaaacc
     4081 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag cttaattaac
     4141 ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     4201 cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
     4261 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
     4321 aagaaggaga tatacatatg accaaggcag atattggaac accaagtaat ttccagcaca
     4381 ttggacatgt tggttgggac ccaaatacag gttttgatct aaataattta gatccagaat
     4441 tgaagaatct ttttgatatg tgtgggatct ctgaggccca gcttaaagac agagaaacat
     4501 caaaagttat ttatgacttt attgaaaaaa caggaggtgt agaagctgtt aaaaatgaac
     4561 tccgaaggca agcaccagga tctggtagtg gctctggagg atctatggca gagtatgtgc
     4621 gggccctgtt tgactttaat gggaatgatg aagaagacct tccctttaag aaaggagaca
     4681 tcctgagaat ccgggataag cctgaagagc agtggtggaa tgcagaggac agcgaaggaa
     4741 agagggggat gattcctgtc ccttacgtgg agaagtatgg atctggtagt ggctctggag
     4801 gatctatgct ccagcggcgc cgcgtgacgg tgcgcaaggc cgacgccggc gggctgggca
     4861 tcagcatcaa ggggggccgg gaaaacaaga tgcctattct catttccaaa atcttcaagg
     4921 gactggcagc agaccagacg gaggcccttt ttgttgggga tgccatcctg tctgtgaatg
     4981 gtgaagattt gtcctctgcc acccacgatg aagcggtaca ggccctcaag aagacaggca
     5041 aggaggttgt gttggaggtt aagtacatga aggaggtctc accctatttc aagggatctg
     5101 gtagtggctc tggaggatct atgctccagc ggcgccgcgt gacggtgcgc aaggccgacg
     5161 ccggcgggct gggcatcagc atcaaggggg gccgggaaaa caagatgcct attctcattt
     5221 ccaaaatctt caagggactg gcagcagacc agacggaggc cctttttgtt ggggatgcca
     5281 tcctgtctgt gaatggtgaa gatttgtcct ctgccaccca cgatgaagcg gtacaggccc
     5341 tcaagaagac aggcaaggag gttgtgttgg aggttaagta catgaaggag gtctcaccct
     5401 atttcaaggg atctggtagt ggctctggag gatctatgct ccagcggcgc cgcgtgacgg
     5461 tgcgcaaggc cgacgccggc gggctgggca tcagcatcaa ggggggccgg gaaaacaaga
     5521 tgcctattct catttccaaa atcttcaagg gactggcagc agaccagacg gaggcccttt
     5581 ttgttgggga tgccatcctg tctgtgaatg gtgaagattt gtcctctgcc acccacgatg
     5641 aagcggtaca ggccctcaag aagacaggca aggaggttgt gttggaggtt aagtacatga
     5701 aggaggtctc accctatttc aagggatctg gtagtggctc tggaggatct atgctccagc
     5761 ggcgccgcgt gacggtgcgc aaggccgacg ccggcgggct gggcatcagc atcaaggggg
     5821 gccgggaaaa caagatgcct attctcattt ccaaaatctt caagggactg gcagcagacc
     5881 agacggaggc cctttttgtt ggggatgcca tcctgtctgt gaatggtgaa gatttgtcct
     5941 ctgccaccca cgatgaagcg gtacaggccc tcaagaagac aggcaaggag gttgtgttgg
     6001 aggttaagta catgaaggag gtctcaccct atttcaaggg atcctgactc gagtctggta
     6061 aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact agtcgcagct
     6121 taattaacct aaactgctgc caccgctgag caataactag cataacccct tggggcctct
     6181 aaacgggtct tgaggggttt tttgctagcg aaaggaggag tcgactatat ccggattggc
     6241 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6301 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6541 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6601 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6661 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttctggcgg cacgatggca
     6721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7681 tcttcctttt tcaatcatga ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7741 atatttgaat gtatttagaa aaataaacaa ataggtcatg accaaaatcc cttaacgtga
     7801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7981 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     8641 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc
     8701 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     8761 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8821 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8881 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     8941 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     9001 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     9061 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     9121 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     9181 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     9241 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     9301 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     9361 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     9421 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     9481 agcctagccg ggtcctcaac gacaggagca cgatcatgct agtcatgccc cgcgcccacc
     9541 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat
     9601 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9721 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     9781 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     9841 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     9901 cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     9961 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
    10021 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
    10081 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
    10141 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
    10201 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
    10261 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
    10321 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
    10381 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
    10441 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
    10501 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
    10561 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
    10621 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
    10681 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
    10741 tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc
    10801 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
    10861 gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
    10921 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
    10981 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
    11041 gccggtgatg ccggccacga tgcgtccggc gtagcctagg atcgagatcg atctcgatcc
    11101 cgcgaaatta atacgactca ctata
//
