LOCUS       sequence-103511-         385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses yellow fluorescent protein , EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_CAG:EYFP
FEATURES             Location/Qualifiers
     source          1..385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..278)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actttgtaca agaaagctgg gtattacttg tacagctcgt ccatgccgag agtgatcccg
       61 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      121 agcttggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      181 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      241 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
      301 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      361 cccttcagct cgatgcggtt cacca
//
