LOCUS       sequence-103513-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of EBFP2 with rtTA for input fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_TRE:EBFP2
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(86..799,800..802,803..805))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     739..760
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      802..811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttcacgcgta
       61 ccactttgta caagaaagct gggtattact tgtacagctc gtccatgccg agagtgatcc
      121 cggcggcggt gcggaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      181 tcagcacgga ctgggtgctc aggtagtggc tgtcgggcag cagcacgggg ccgtcgccga
      241 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcc acgttgtggc
      301 ggatcttgaa gttcaccttg atgccgttct tctgcttgac ggccatgata tagatgttgt
      361 ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      421 cgcccttcag ctcgatgcgg ttcactaggg tgtcgccctc gaacttcacc tcggcgcggg
      481 tcttgtaggt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      541 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca
      601 cgccgtggct cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      661 agatgaactt cagggtcagc ttgccgttgg tggcatcgcc ctcgccctcg cccctcacgc
      721 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      781 acagctcctc gcccttgctc accatggtgg cggtcagcct gctt
//
