LOCUS       sequence-103519-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and sgRNA scaffold module for gRNA expression. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        complement(112..187)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(304..323)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tatagaaaag ttgggtgcga attcaaaaaa agcaccgact
      121 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
      181 ctaaaacgtc agcaggttct tgatccgtcg agattttcag gagctaagga agctaaaatg
      241 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      301 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      361 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      421 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
      481 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      541 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      601 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      661 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      721 aatatggaca acttcttcg
//
