LOCUS       sequence-103520-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV tdTomato L5-L2
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..78
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        134..535
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      545..554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(684..702)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtatac aaaagttggg agacccaagc ttcgctgaaa gaccccacct gtaggtttgg
      121 caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga
      181 gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct
      241 gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc
      301 cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt
      361 aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt
      421 gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta
      481 taaaagagct cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgccgga
      541 tcccgccacc atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt
      601 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
      661 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
      721 cgcctgggac atcctgt
//
