LOCUS       sequence-103523-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Duet vector for co-expression of GFP and mCherry from PBAD and 
            PLtetO promoters, respectively; parent vector for split mCherry 
            assembly vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Sawyer N, Gassaway BM, Haimovich AD, Isaacs FJ, Rinehart J, Regan L
  TITLE     Designed Phosphoprotein Recognition in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Nov 21;9(11):2502-7. doi: 10.1021/cb500658w. 
            Epub 2014 Oct 6.
  PUBMED    25272187
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500658w";
            journalName: "ACS Chem Biol"; date: "2014-11-21- 21"; volume: "9"; 
            issue: "11"; pages: "2502-7"
COMMENT     Sequence Label: pNAS1B
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(194..212,213..218,219..235,236..241,242..267)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    194..212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             277..288
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(446..464)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     714..733
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
       61 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      181 tgagcgaaga tcttccctat cagtgataga gattgacatc cctatcagtg atagagatac
      241 tgagcacatc agcaggacgc actgaccgaa ttcattaaag aggagaaagg taccatggca
      301 tccgtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      361 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      421 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      481 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      541 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      601 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      661 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      721 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      781 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcc
//
