LOCUS       sequence-103527-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of TAL21 as input repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_TRE:TAL21
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(197..217)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttcacgcgta
       61 ccactttgta caagaaagct gggtagatca gttatctaga tccggtggat tttcagttac
      121 ccgggggtac cttaggcgcg ccgggccctt aactagttga gccgccacca agctttgagc
      181 cgccaccgct ggcctccacc tttctcttct tcttggggct gggatccggg aggccgcccc
      241 agatcctggt acgcgggcgt tttaccctcc agctgagggg caaatgcagc gcatcgcgct
      301 gttcgggaac gcgcacctcg acagcctgct gtgcggaggg gccggtgaca gcacgatccg
      361 atcgggaccg tttacggctg cttgcccgcg tctgatctcc ctcgtgcatt gggctgggcg
      421 catcaaggtc acgctccagc gaatcggcga atgcatgcaa agacgcctga tccggtgttt
      481 gagctgaagt aggggacggt ttggcccttt tcatccctga tgcctggagg atacggtccc
      541 aacgctgcga ggctgggggg agcgttccac cgcgggcttc gagttcggtg acgcccactc
      601 tgcgaaagag ctgtaccaac ccgttcctgc tcatcccgaa ctgcgtcatg gcctcatcaa
      661 atgcgtacgc tgggtgggag tggcactgga aaaactccag cacgcgaacc acttgcgcgt
      721 agtc
//
