LOCUS       sequence-103534-       14761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 
            Ratio).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, 
            Wang Z, Koffas MA
  TITLE     Improvement of catechin production in Escherichia coli through 
            combinatorial metabolic engineering.
  JOURNAL   Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
  PUBMED    25527438
REFERENCE   2  (bases 1 to 14761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2014-12-17"; volume: "28C:43-53. doi"; pages: " 
            10.1016/j.ymben.2014.12.002"
COMMENT     Sequence Label: p1585588
FEATURES             Location/Qualifiers
     source          1..14761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             219..230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1287..1331
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1384..1431
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1484..1508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1523..1545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2642..2686
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2739..2786
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2820..2838
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2839..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2878..2900
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4090..4134
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4187..4234
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4268..4286
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4287..4311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4326..4348
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5445..5489
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5542..5589
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5623..5641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5642..5666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5681..5703
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6800..6844
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      6897..6944
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6978..6996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6997..7021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7036..7058
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8248..8292
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      8345..8392
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        8426..8444
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8445..8469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8484..8506
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9696..9740
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      9793..9840
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      9884..10339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     10181..10202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(10454..11245,11246..11314))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11077..11096
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11315..11407)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11488..12076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11977..11996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12230..12247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12262..12404
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12330..12349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12490..12512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12506..12697)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(13269..14351)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     14313..14332
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(14352..14429)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     14635..14654
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(14745..14761,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
      121 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
      181 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
      241 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
      301 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
      361 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
      421 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
      481 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
      541 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
      601 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
      661 aagtggtgat caacttctat ccgaaatgtc cgcagccgga tctgaccctg ggtctgaaac
      721 gtcataccga tccgggtaca attaccctgc tgctgcaaga tcaggtgggt ggtctgcagg
      781 caacccgtga tggtggcaaa acctggatta ccgttcagcc ggttgaaggt gcatttgttg
      841 ttaatctggg tgatcatggc cattatctga gcaatggtcg ctttaaaaac gcagatcatc
      901 aggcagttgt taatagcaat tgtagccgtc tgagcattgc aacctttcag aatccggcac
      961 cggaagcaac cgtttatccg ctgaaaattc gtgaaggtga aaaaccgatt ctggaagaac
     1021 cgattacctt tgccgatatg tataaacgca aaatgagcaa agatatcgag ctggccaaac
     1081 tgaaaaaact ggcgaaagaa aaaaaactgc tgcaagacca gcaggatatc gaaaaagcaa
     1141 aactggaaat caaaagcacc gatgaaatct tcgccctggt tggtgcactg atgcatgtta
     1201 tgcagaaacg tagccgtgca attcatagca gtgatgaagg tgaagatcaa gccggtgatg
     1261 aagatgagga ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
     1321 acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa
     1381 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctaggatcg
     1441 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     1501 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgaaaga
     1561 tagcgttgca agcgcaaccg caagcgcacc gggtacagtt tgtgttaccg gtgcagcagg
     1621 ttttattggt agctggctgg ttatgcgtct gctggaacgt ggttatattg ttcgtgcaac
     1681 cgttcgtgat ccggcaaatc tgaaaaaagt taaacatctg ctggatctgc cgaaagcaga
     1741 taccaatctg accctgtgga aagccgatct gaatgaagag ggtagctttg atgaagcaat
     1801 tgaaggttgt agcggtgttt ttcatgttgc aaccccgatg gattttgaaa gcaaagatcc
     1861 ggaaaacgaa gtgattaaac cgaccattaa cggtgtgctg agcattattc gtagctgtac
     1921 caaagcaaaa accgttaaac gtctggtttt taccagcagc gcaggtacag ttaatgttca
     1981 agaacatcag cagccggtgt ttgatgaaaa caattggagc gatctgcact tcatcaacaa
     2041 aaaaaaaatg accggctgga tgtattttgt gagcaaaacc ctggcagaaa aagcagcatg
     2101 ggaagcagca aaagaaaaca acattgattt catcagcatt atcccgaccc tggttggtgg
     2161 tccgtttatt atgccgacct ttccgcctag cctgattacc gcactgagcc cgattacccg
     2221 taatgaaggt cattattcca ttatcaaaca gggccagttt gtgcatctgg atgatctgtg
     2281 tgaaagccac atttttctgt atgaacgtcc gcaggcagaa ggtcgttata tttgtagcag
     2341 ccatgatgca accattcatg atctggccaa actgatgcgt gaaaaatggc ctgaatataa
     2401 tgttccgacc gaattcaaag gcatcgataa agatctgccg gttgttagct tttccagcaa
     2461 aaaactgatt ggcatgggct tcgagttcaa atatagcctg gaagatatgt ttcgtggtgc
     2521 cattgatacc tgtcgtgaaa aaggtctgct gccgcatagc tttgcagaaa atccggttaa
     2581 tggcaacaaa gtgcctcctc ctgcactgcc tccgaaacgt cgtcgttaac tcgagtctgg
     2641 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     2701 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     2761 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     2821 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     2881 gtttaacttt aagaaggaga tatacatatg accgttagcg gtgcaattcc gagcatgacc
     2941 aaaaatcgta ccctggttgt tggtggcacc ggttttattg gtcagtttat taccaaagca
     3001 agcctgggtt ttggttatcc gacctttctg ctggttcgtc cgggtccggt tagcccgagc
     3061 aaagcagtta ttatcaaaac ctttcaggat aaaggtgcca aagtgattta tggcgtgatc
     3121 aacgataaag aatgcatgga aaaaattctg aaagagtacg agatcgacgt tgttattagc
     3181 ctggtgggtg gtgcacgtct gctggatcag ctgaccctgc tggaagcaat taaaagcgtt
     3241 aaaaccatca aacgttttct gccgagcgaa tttggccatg atgttgatcg taccgatccg
     3301 gttgaaccgg gtctgaccat gtataaagaa aaacgtctgg tgcgtcgtgc cgttgaagaa
     3361 tatggtattc cgtttaccaa tatctgctgc aatagcattg caagctggcc gtattatgat
     3421 aattgtcatc cgagccaggt tccgcctccg atggatcagt ttcagattta tggtgatggt
     3481 aacaccaaag cctatttcat tgatggcaac gatatcggca aatttaccat gaaaaccatc
     3541 gatgatattc gcaccctgaa caaaaatgtt cattttcgtc cgagcagcaa ctgctacagc
     3601 attaatgaac tggcaagcct gtgggagaaa aaaatcggtc gtacactgcc tcgttttacc
     3661 gttaccgcag ataaactgct ggcacatgca gcagaaaaca ttattccgga aagcattgtt
     3721 agcagcttta cccacgatat ctttattaac ggttgccagg tgaactttag catcgatgaa
     3781 catagtgatg tggaaatcga tacactgtat ccggatgaaa aatttcgtag cctggatgat
     3841 tgctatgaag attttgttcc gatggtgcac gataaaattc atgcaggtaa aagcggtgaa
     3901 atcaaaatca aagatggtaa accgctggtt cagaccggca ccattgaaga aattaacaaa
     3961 gacattaaaa ccctggtgga aacccagccg aatgaagaga tcaaaaaaga tatgaaagca
     4021 ctggttgaag ccgttccgat tagcgcaatg ggtggtgtta aagaaagcct ggtttaactc
     4081 gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact
     4141 agtcgcagct taattaacct aaactgctgc caccgctgag caataactag cataacccct
     4201 tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga tctcgatccc
     4261 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
     4321 ataattttgt ttaactttaa gaaggagata tacatatgaa agatagcgtt gcaagcgcaa
     4381 ccgcaagcgc accgggtaca gtttgtgtta ccggtgcagc aggttttatt ggtagctggc
     4441 tggttatgcg tctgctggaa cgtggttata ttgttcgtgc aaccgttcgt gatccggcaa
     4501 atctgaaaaa agttaaacat ctgctggatc tgccgaaagc agataccaat ctgaccctgt
     4561 ggaaagccga tctgaatgaa gagggtagct ttgatgaagc aattgaaggt tgtagcggtg
     4621 tttttcatgt tgcaaccccg atggattttg aaagcaaaga tccggaaaac gaagtgatta
     4681 aaccgaccat taacggtgtg ctgagcatta ttcgtagctg taccaaagca aaaaccgtta
     4741 aacgtctggt ttttaccagc agcgcaggta cagttaatgt tcaagaacat cagcagccgg
     4801 tgtttgatga aaacaattgg agcgatctgc acttcatcaa caaaaaaaaa atgaccggct
     4861 ggatgtattt tgtgagcaaa accctggcag aaaaagcagc atgggaagca gcaaaagaaa
     4921 acaacattga tttcatcagc attatcccga ccctggttgg tggtccgttt attatgccga
     4981 cctttccgcc tagcctgatt accgcactga gcccgattac ccgtaatgaa ggtcattatt
     5041 ccattatcaa acagggccag tttgtgcatc tggatgatct gtgtgaaagc cacatttttc
     5101 tgtatgaacg tccgcaggca gaaggtcgtt atatttgtag cagccatgat gcaaccattc
     5161 atgatctggc caaactgatg cgtgaaaaat ggcctgaata taatgttccg accgaattca
     5221 aaggcatcga taaagatctg ccggttgtta gcttttccag caaaaaactg attggcatgg
     5281 gcttcgagtt caaatatagc ctggaagata tgtttcgtgg tgccattgat acctgtcgtg
     5341 aaaaaggtct gctgccgcat agctttgcag aaaatccggt taatggcaac aaagtgcctc
     5401 ctcctgcact gcctccgaaa cgtcgtcgtt aactcgagtc tggtaaagaa accgctgctg
     5461 cgaaatttga acgccagcac atggactcgt ctactagtcg cagcttaatt aacctaaact
     5521 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     5581 ggttttttgc taggatcgag atcgatctcg atcccgcgaa attaatacga ctcactatag
     5641 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
     5701 agatatacat atgaaagata gcgttgcaag cgcaaccgca agcgcaccgg gtacagtttg
     5761 tgttaccggt gcagcaggtt ttattggtag ctggctggtt atgcgtctgc tggaacgtgg
     5821 ttatattgtt cgtgcaaccg ttcgtgatcc ggcaaatctg aaaaaagtta aacatctgct
     5881 ggatctgccg aaagcagata ccaatctgac cctgtggaaa gccgatctga atgaagaggg
     5941 tagctttgat gaagcaattg aaggttgtag cggtgttttt catgttgcaa ccccgatgga
     6001 ttttgaaagc aaagatccgg aaaacgaagt gattaaaccg accattaacg gtgtgctgag
     6061 cattattcgt agctgtacca aagcaaaaac cgttaaacgt ctggttttta ccagcagcgc
     6121 aggtacagtt aatgttcaag aacatcagca gccggtgttt gatgaaaaca attggagcga
     6181 tctgcacttc atcaacaaaa aaaaaatgac cggctggatg tattttgtga gcaaaaccct
     6241 ggcagaaaaa gcagcatggg aagcagcaaa agaaaacaac attgatttca tcagcattat
     6301 cccgaccctg gttggtggtc cgtttattat gccgaccttt ccgcctagcc tgattaccgc
     6361 actgagcccg attacccgta atgaaggtca ttattccatt atcaaacagg gccagtttgt
     6421 gcatctggat gatctgtgtg aaagccacat ttttctgtat gaacgtccgc aggcagaagg
     6481 tcgttatatt tgtagcagcc atgatgcaac cattcatgat ctggccaaac tgatgcgtga
     6541 aaaatggcct gaatataatg ttccgaccga attcaaaggc atcgataaag atctgccggt
     6601 tgttagcttt tccagcaaaa aactgattgg catgggcttc gagttcaaat atagcctgga
     6661 agatatgttt cgtggtgcca ttgatacctg tcgtgaaaaa ggtctgctgc cgcatagctt
     6721 tgcagaaaat ccggttaatg gcaacaaagt gcctcctcct gcactgcctc cgaaacgtcg
     6781 tcgttaactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga
     6841 ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag caataactag
     6901 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga
     6961 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
     7021 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgac cgttagcggt
     7081 gcaattccga gcatgaccaa aaatcgtacc ctggttgttg gtggcaccgg ttttattggt
     7141 cagtttatta ccaaagcaag cctgggtttt ggttatccga cctttctgct ggttcgtccg
     7201 ggtccggtta gcccgagcaa agcagttatt atcaaaacct ttcaggataa aggtgccaaa
     7261 gtgatttatg gcgtgatcaa cgataaagaa tgcatggaaa aaattctgaa agagtacgag
     7321 atcgacgttg ttattagcct ggtgggtggt gcacgtctgc tggatcagct gaccctgctg
     7381 gaagcaatta aaagcgttaa aaccatcaaa cgttttctgc cgagcgaatt tggccatgat
     7441 gttgatcgta ccgatccggt tgaaccgggt ctgaccatgt ataaagaaaa acgtctggtg
     7501 cgtcgtgccg ttgaagaata tggtattccg tttaccaata tctgctgcaa tagcattgca
     7561 agctggccgt attatgataa ttgtcatccg agccaggttc cgcctccgat ggatcagttt
     7621 cagatttatg gtgatggtaa caccaaagcc tatttcattg atggcaacga tatcggcaaa
     7681 tttaccatga aaaccatcga tgatattcgc accctgaaca aaaatgttca ttttcgtccg
     7741 agcagcaact gctacagcat taatgaactg gcaagcctgt gggagaaaaa aatcggtcgt
     7801 acactgcctc gttttaccgt taccgcagat aaactgctgg cacatgcagc agaaaacatt
     7861 attccggaaa gcattgttag cagctttacc cacgatatct ttattaacgg ttgccaggtg
     7921 aactttagca tcgatgaaca tagtgatgtg gaaatcgata cactgtatcc ggatgaaaaa
     7981 tttcgtagcc tggatgattg ctatgaagat tttgttccga tggtgcacga taaaattcat
     8041 gcaggtaaaa gcggtgaaat caaaatcaaa gatggtaaac cgctggttca gaccggcacc
     8101 attgaagaaa ttaacaaaga cattaaaacc ctggtggaaa cccagccgaa tgaagagatc
     8161 aaaaaagata tgaaagcact ggttgaagcc gttccgatta gcgcaatggg tggtgttaaa
     8221 gaaagcctgg tttaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
     8281 cacatggact cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca
     8341 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc
     8401 gagatcgatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     8461 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgaccg
     8521 ttagcggtgc aattccgagc atgaccaaaa atcgtaccct ggttgttggt ggcaccggtt
     8581 ttattggtca gtttattacc aaagcaagcc tgggttttgg ttatccgacc tttctgctgg
     8641 ttcgtccggg tccggttagc ccgagcaaag cagttattat caaaaccttt caggataaag
     8701 gtgccaaagt gatttatggc gtgatcaacg ataaagaatg catggaaaaa attctgaaag
     8761 agtacgagat cgacgttgtt attagcctgg tgggtggtgc acgtctgctg gatcagctga
     8821 ccctgctgga agcaattaaa agcgttaaaa ccatcaaacg ttttctgccg agcgaatttg
     8881 gccatgatgt tgatcgtacc gatccggttg aaccgggtct gaccatgtat aaagaaaaac
     8941 gtctggtgcg tcgtgccgtt gaagaatatg gtattccgtt taccaatatc tgctgcaata
     9001 gcattgcaag ctggccgtat tatgataatt gtcatccgag ccaggttccg cctccgatgg
     9061 atcagtttca gatttatggt gatggtaaca ccaaagccta tttcattgat ggcaacgata
     9121 tcggcaaatt taccatgaaa accatcgatg atattcgcac cctgaacaaa aatgttcatt
     9181 ttcgtccgag cagcaactgc tacagcatta atgaactggc aagcctgtgg gagaaaaaaa
     9241 tcggtcgtac actgcctcgt tttaccgtta ccgcagataa actgctggca catgcagcag
     9301 aaaacattat tccggaaagc attgttagca gctttaccca cgatatcttt attaacggtt
     9361 gccaggtgaa ctttagcatc gatgaacata gtgatgtgga aatcgataca ctgtatccgg
     9421 atgaaaaatt tcgtagcctg gatgattgct atgaagattt tgttccgatg gtgcacgata
     9481 aaattcatgc aggtaaaagc ggtgaaatca aaatcaaaga tggtaaaccg ctggttcaga
     9541 ccggcaccat tgaagaaatt aacaaagaca ttaaaaccct ggtggaaacc cagccgaatg
     9601 aagagatcaa aaaagatatg aaagcactgg ttgaagccgt tccgattagc gcaatgggtg
     9661 gtgttaaaga aagcctggtt taactcgagt ctggtaaaga aaccgctgct gcgaaatttg
     9721 aacgccagca catggactcg tctactagtc gcagcttaat taacctaaac tgctgccacc
     9781 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     9841 ctagcgaaag gaggagtcga ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
     9901 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9961 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    10021 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    10321 atattaacgt ttacaatttc tggcggcacg atggcatgag attatcaaaa aggatcttca
    10381 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10441 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10501 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10561 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10621 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10681 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10741 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10801 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10861 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10921 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10981 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11041 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11101 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11161 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11221 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11281 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatgattga
    11341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11401 aaacaaatag gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    11641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    11941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    12001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    12241 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
    12301 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    12361 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
    12421 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
    12481 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
    12541 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
    12601 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
    12661 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
    12721 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
    12781 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
    12841 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
    12901 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
    12961 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
    13021 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
    13081 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
    13141 ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
    13201 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
    13261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    13321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
    13381 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
    13441 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
    13501 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca
    13561 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
    13621 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
    13681 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
    13741 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
    13801 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
    13861 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
    13921 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
    13981 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
    14041 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
    14101 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
    14161 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
    14221 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
    14281 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
    14341 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
    14401 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
    14461 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
    14521 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
    14581 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
    14641 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
    14701 tccggcgtag cctaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
    14761 a
//
