LOCUS       sequence-103538-         342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and a non-silencing (scrambled) shRNA module. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 n.s shRNA L5-L2
FEATURES             Location/Qualifiers
     source          1..342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..48
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        91..339
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     91..111
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     262..281
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ttgatgagca atgctttttt ataatgccaa ctttgtatac aaaagttgct atcgatccga
       61 gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat
      121 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
      181 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
      241 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
      301 ttcttggctt tatatatctt gtggaaagga cgaaacaccg gt
//
