LOCUS       sequence-103543-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and firefly luciferase module. Compatible with MultiSite 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgcagtacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaactttat tatacaaagt tgtcctctag aattattaca
      181 cggcgatctt gccgcccttc ttggccttaa tgagaatctc gcggatcttg cgggcgtcca
      241 acttgccggt cagtccttta ggcacctcgt ccacgaacac aacaccaccg cgcagcttct
      301 tggcggttgt aacctggctg gccacatagt ccacgatctc cttctcggtc atggttttac
      361 cgtgttccag cacgacgact gcggcgggca gctcgccggc atcgtcgtcg ggcaggccgg
      421 cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat gctctccagt tcggctgggg
      481 ctacctggta gcccttgtat ttgatcaggc tcttcagccg gtccacgatg aagaagtgct
      541 cgtcctcgtc ccagtaggcg atgtcgccgc tgtgcagcca gccgtccttg tcgatgagag
      601 cgtttgtagc ctcggggttg ttaacgtagc cgctcatgat catggggcca cggacgcaca
      661 gctcgccgcg ctggttcaca cccagtgtct taccggtgtc caagtccacc accttagcct
      721 c
//
