LOCUS       sequence-103546-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and firefly luciferase module. Compatible with MultiSite 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtacctcta
      121 gaattattac acggcgatct tgccgccctt cttggcctta atgagaatct cgcggatctt
      181 gcgggcgtcc aacttgccgg tcagtccttt aggcacctcg tccacgaaca caacaccacc
      241 gcgcagcttc ttggcggttg taacctggct ggccacatag tccacgatct ccttctcggt
      301 catggtttta ccgtgttcca gcacgacgac tgcggcgggc agctcgccgg catcgtcgtc
      361 gggcaggccg gcgaccccgg cgtcgaagat gttggggtgt tgcagcagga tgctctccag
      421 ttcggctggg gctacctggt agcccttgta tttgatcagg ctcttcagcc ggtccacgat
      481 gaagaagtgc tcgtcctcgt cccagtaggc gatgtcgccg ctgtgcagcc agccgtcctt
      541 gtcgatgaga gcgtttgtag cctcggggtt gttaacgtag ccgctcatga tcatggggcc
      601 acggacgcac agctcgccgc gctggttcac acccagtgtc ttaccggtgt ccaagtccac
      661 caccttagcc tcgaag
//
