LOCUS       sequence-103549-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and 
            Mxi1 domain controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9-Mxi1
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..147)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(170..836)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(277..300)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(885..904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(886..904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(911..928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(911..927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(920..942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atgccggtcg gtgttcccga gcaccttgaa cttcttagag ggcaccttgt actcgtcagt
       61 gatcacggcc cacccgacgc tattagtccc gatggccagt ccgatagaat acttcttgtc
      121 gctgcctacc tttctcttct tctttgggac gtccatggtg gcggcgggtt ttgtttgttt
      181 atgtgtgttt attcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa gactaactat
      241 aaaagtagaa tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
      301 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt
      361 ttttcagctt tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
      421 tagatacatg cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac
      481 tccattgagg ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta
      541 agagaatgga cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc
      601 tttttttttc tggatgccag cttaaaaagc gggctccatt atatttagtg gatgccagga
      661 ataaactgtt cacccagaca cctacgatgt tatatattct gtgtaacccg ccccctattt
      721 tgggcatgta cgggttacag cagaattaaa aggctaattt tttgactaaa taaagttagg
      781 aaaatcacta ctattaatta tttacgtatt ctttgaaatg gcagtattga taatgataaa
      841 ctcgaactga ctagtagact gaattccgag tgagctccaa ttcgccctat agtgagtcgt
      901 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      961 aacttaatcg ccttgcagca catccccc
//
