LOCUS       sequence-103551-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L1-R5
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..151)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(381..595)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(441..460)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(643..667)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactaatac catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat
       61 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      121 gtttctcgtt caacttttgt atacaaagtt gtcgaattca aaaaagagct cggactcggg
      181 cgccggcctg gagctgggca tcgcgtcctc cccgacgcct ggctccaccg cgtcgacggg
      241 cggcaaggcg gacgacccca gctggtgcaa gacgcccagt ggccacatca agcggcccat
      301 gaacgccttt atggtgtggt cgcagatcga gcggcgcaag atcatggagc agtcgcccga
      361 catgcacaac gccgagatct gtggtctcat acagaactta taagattccc aaatccaaag
      421 acatttcacg tttatggtga tttcccagaa cacatagcga catgcaaata ttgcagggcg
      481 ccactcccct gtccctcaca gccatcttcc tgccagggcg cacgcgcgct gggtgttccc
      541 gcctagtgac actgggcccg cgattccttg gagcgggttg atgacgtcag cgttcgaatt
      601 cctgcagccc gggggatcca gtcgactgaa ttggatcgat agagcctgct tttttgtaca
      661 aagttggcat tataaaaaag cattgctcat caatttgttg caacgaacag gtcactatca
      721 gtc
//
