LOCUS       sequence-103553-         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1 L5-L4
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..110
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(343..433)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(403..422)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtat agaaaagttg ggtgcgaatt
      121 caaaaaagag ctcggactcg ggcgccggcc tggagctggg catcgcgtcc tccccgacgc
      181 ctggctccac cgcgtcgacg ggcggcaagg cggacgaccc cagctggtgc aagacgccca
      241 gtggccacat caagcggccc atgaacgcct ttatggtgtg gtcgcagatc gagcggcgca
      301 agatcatgga gcagtcgccc gacatgcaca acgccgagat ctgtggtctc atacagaact
      361 tataagattc ccaaatccaa agacatttca cgtttatggt gatttcccag aacacatagc
      421 gacatgcaaa tattgcaggg cgccactccc ctgtccctca cagccatctt cctgccaggg
      481 cgcacgcgcg ctgggtgtt
//
