LOCUS       sequence-103555-         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 L5-L2
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..113
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(219..548)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      233..368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     533..553
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtac aagaaagctg ggtagtaatc
      121 tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattcc agcacactgg
      181 cggccgttac tagtggatcc gagctcggta ccaagctttt tgcaaaagcc taggcctcca
      241 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
      301 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
      361 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
      421 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
      481 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
      541 tgacacacat tccacagcaa cttttg
//
