LOCUS       sequence-103557-         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV L1-R5
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        137..538
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tggagaccca agcttcgctg aaagacccca cctgtaggtt
      121 tggcaagcta gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat
      181 agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata
      241 tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc
      301 ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt
      361 cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta
      421 tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct
      481 ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc
      541 ggatccacta gtaacggccg ccagtgtgct ggaattctgc agatatccat cacactggcg
      601 g
//
