LOCUS       sequence-103561-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and multiple cloning site flanked by loxP sites. Compatible
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV loxP L5-L2
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    104..137
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    229..262
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(276..295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(277..295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(340..543)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(369..389)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
       61 ttataatgcc aactttgtac aagaaagctg ggtacctcta gacataactt cgtataatgt
      121 atgctatacg aagttatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt
      181 ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagcat aacttcgtat
      241 aatgtatgct atacgaagtt atgaagcttg ggtctcccta tagtgagtcg tattaatttc
      301 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
      361 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
      421 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
      481 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
      541 caccatggta atagcgatga ctaatacgta
//
