LOCUS       sequence-103567-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6-miR-30 L1-L4
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        146..394
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     146..166
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     317..336
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           449..542
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           556..668
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(693..713)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tggcaaaacg caccacgtga cggagcgtga ccgcgcgccg
      121 agcgcgcgcc agggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca
      181 tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat
      241 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
      301 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      361 ggctttatat atcttgtgga aaggacgaaa caccgtgctc gcttcggcag cacatatact
      421 agtcgactag ggataacagg gtaattgttt gaatgaggct tcagtacttt acagaatcgt
      481 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg
      541 ctcgagcaac cagaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg
      601 aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
      661 atcacttttt tcaattggaa gactaatgca cccaactttt ctatacaaag ttggcattat
      721 aagaaagcat tgcttatcaa tttgttgcaa cg
//
