LOCUS       sequence-103568-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6-miR-30 L1-L4
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..110
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(135..247)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(261..354)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(409..657)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(467..486)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(637..657)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtat agaaaagttg ggtgcattag
      121 tcttccaatt gaaaaaagtg atttaattta taccatttta attcagcttt gtaaaaatgt
      181 atcaaagaga tagcaaggta ttcagtttta gtaaacaaga taattgctcc taaagtagcc
      241 ccttgaattc tggttgctcg agccttctgt tgggttaacc tgaagaagta atcccagcaa
      301 gtgtttccaa gatgtgcagg caacgattct gtaaagtact gaagcctcat tcaaacaatt
      361 accctgttat ccctagtcga ctagtatatg tgctgccgaa gcgagcacgg tgtttcgtcc
      421 tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg
      481 atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct
      541 acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc
      601 tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcttc
      661 ctgcccgacc ctggcgcgcg ctcggcgcgc ggtcacgctc cgtcacgt
//
