LOCUS       sequence-103572-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 
            tet-inducible CMV promoter and miR30-based hairpin module for shRNA 
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV/TO-miR-30 L1-L4
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     ncRNA           complement(132..244)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(258..351)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    389..407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(430..633)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(459..479)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tatagaaaag ttgggtgcct ctagataaac gcattagtct
      121 tccaattgaa aaaagtgatt taatttatac cattttaatt cagctttgta aaaatgtatc
      181 aaagagatag caaggtattc agttttagta aacaagataa ttgctcctaa agtagcccct
      241 tgaattctgg ttgctcgagc cttctgttgg gttaacctga agaagtaatc ccagcaagtg
      301 tttccaagat gtgcaggcaa cgattctgta aagtactgaa gcctcattca aacaattacc
      361 ctgttatccc tagtcgacta gtcgacgatc tctatcactg atagggagat ctctatcact
      421 gatagggaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      481 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
      541 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
      601 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
      661 gatgtactgc caagta
//
