LOCUS       sequence-103576-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and EGFP module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 eGFP L5-L2
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..78
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(83..103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        88..417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      268..403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      446..455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(497..518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtatac aaaagttgct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      421 cttggtaccg agctcggatc ctaaagccac catggtgagc aagggcgagg agctgttcac
      481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatg
//
