LOCUS       sequence-103581-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 
            tet-inducible CMV promoter and miR30-based hairpin module for shRNA 
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV/TO-miR-30 L5-L2
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(160..272)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(286..379)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    417..435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    438..456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(458..661)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(487..507)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgcggccgcc ctgcagctgg atggcaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt cttataatgc caactttgta caagaaagct
      121 gggtacctct agataaacgc attagtcttc caattgaaaa aagtgattta atttatacca
      181 ttttaattca gctttgtaaa aatgtatcaa agagatagca aggtattcag ttttagtaaa
      241 caagataatt gctcctaaag tagccccttg aattctggtt gctcgagcct tctgttgggt
      301 taacctgaag aagtaatccc agcaagtgtt tccaagatgt gcaggcaacg attctgtaaa
      361 gtactgaagc ctcattcaaa caattaccct gttatcccta gtcgactagt cgacgatctc
      421 tatcactgat agggagatct ctatcactga tagggagagc tctgcttata tagacctccc
      481 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      541 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      601 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      661 ccatggtaat agcgatgact aatacgtaga tgtactg
//
