LOCUS       sequence-103591-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and firefly luciferase module. Compatible with MultiSite 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 Luc2 (FF-Luciferase) L5-L2
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtagta atctagaatt attacacggc
      121 gatcttgccg cccttcttgg ccttaatgag aatctcgcgg atcttgcggg cgtccaactt
      181 gccggtcagt cctttaggca cctcgtccac gaacacaaca ccaccgcgca gcttcttggc
      241 ggttgtaacc tggctggcca catagtccac gatctccttc tcggtcatgg ttttaccgtg
      301 ttccagcacg acgactgcgg cgggcagctc gccggcatcg tcgtcgggca ggccggcgac
      361 cccggcgtcg aagatgttgg ggtgttgcag caggatgctc tccagttcgg ctggggctac
      421 ctggtagccc ttgtatttga tcaggctctt cagccggtcc acgatgaaga agtgctcgtc
      481 ctcgtcccag taggcgatgt cgccgctgtg cagccagccg tccttgtcga tgagagcgtt
      541 tgtagcctcg gggttgttaa cgtagccgct catgatcatg gggccacgga cgcacagctc
      601 gccgcgctgg ttcacaccca gtgtcttacc ggtgtccaag tccaccacct tagcctcgaa
      661 g
//
