LOCUS       sequence-103614-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 tdTomato L5-L2
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     112..128
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..258)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(433..452)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtagta atctagaggc tcgaggtcga
      121 cggtatcgat aagcttgata tcgaattctt acttgtacag ctcgtccatg ccgtacagga
      181 acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag tcctcgttgt
      241 gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagttgc acgggcttct
      301 tggccatgta gatggtcttg aactccacca ggtagcggcc gccgtccttc agcttcaggg
      361 cctggtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggaggcct
      421 cccagcccat ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg gtgccgcgca
      481 tcttcacctt gtagatcagc gtgccgtcct gcagggagga gtcctgggtc acggtcacca
      541 gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      601 tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc cttggagccg tacatgaact
      661 ggggggacag gatgtccca
//
