LOCUS       sequence-103617-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-WASH1-N-18
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             498..506
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg gcgaccggtg
      121 gatcccgggc ccgcggtacc gtcgactgca gaattccgct ggactcccag tcctcctcat
      181 cctcgtctcc tgccggctgc tgtgggggtg gcagcggtgg gatggagtct gacattcgag
      241 agaaggctcc tccaggtccc tcactggttc cagtgctagg tccctttcca gagatacctt
      301 tgcgcctcat gacaagtttg ttgaagagat ctgacatcaa gtccccacct tggctcgtgg
      361 ctctcacttg ctcctgctcc ttctgtttct tcttctccag tttgcgctcc ttcacgctgc
      421 gtagcttggc cttgccaatg cccccagctt ggcggatgga ctcgagcaga gtggcccggc
      481 cactggaggg gtcaaccacc tccttagggg ctccctgaac agaggctgtg tgggccatgc
      541 tgctgctaga gtcttcttcc ctggcaacct ggccagcagc agttgccatg tcagggaaca
      601 tcggtggctg tggggtactg accaatgctg tgggagctgg gggaggtggc ggcggtgggg
      661 gaggaggcgg gggcggtgct gccaacagta cttcattctc tagctctggc tggagaggtt
      721 ctgccacctc tgtgtggaag gcaggcaact ctgggatagc accaggagca gacggggcaa
      781 tgccagggcc caaatccgca ctgtacatca ggtcgtctgc gacgccgggt aaatcgggta
      841 ggtaggatgg cacgtcgatc tcaggaacct ggc
//
