LOCUS       sequence-103633-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving human Ngn2;
            constitutive expression of Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_hNgn2_UBC_Puro
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..34
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    79..97
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    114..132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    150..168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    221..239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    257..275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        287..325
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     322..346
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    365..389
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgctagtga tatcaacttt gtatagaaaa gttggctccg aattcgccct tcaggtccga
       61 ggttctagac gagtttactc cctatcagtg atagagaacg atgtcgagtt tactccctat
      121 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
      181 tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag tgatagagaa
      241 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg
      301 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcaa agggcgaatt
      361 cgacccaagt ttgtacaaaa aagcaggctg acctccacca tgttcgtcaa atccgagacc
      421 ttggagttga aggaggaaga ggacgtgtta gtgctgctcg gatcggcctc ccccgccttg
      481 gcggccctga ccccgctgtc atccagcgcc gacgaagaag aggaggagga gccgggcgcg
      541 tcaggcgggg cgcgtcggca gcgcggggct gaggccgggc agggggcgcg gggcggcgtg
      601 gctgcgggtg cggagggctg ccggcccgca cggctgctgg gtctggtaca cgattgcaaa
      661 cggcgccctt cccgggcgcg ggccgtctcc cgaggcgcca agacggccga gacggtgcag
      721 cgcatcaaga agacccgtag actgaaggcc aacaaccgcg agcgaaaccg catgcacaac
      781 ctcaacgcgg cactggacgc gctgcgcgag gtgctcccca cgttccccga ggacgccaag
      841 ctcaccaaga tcgagacc
//
