LOCUS       sequence-103640-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 
            and Ngn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_Ngn2‐2A‐Ngn1
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..73
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattgc cggaattcac cactttgtac
       61 aagaaagctg ggtactagtg gtatgggatg aaacagggcg tcgtgtggag caggtctttg
      121 ggcaggccag gaaaggagaa aaggggatcg cccgggccat aggtgaagtc ttctgaagcc
      181 gagggactac tggggtcaga gagtggtgat gccacagtgg cgcaggggga ggcagcggcc
      241 ccggaacccc aggactcagt gtcgctggcc gggctcgggg gcccgggcag acaggggaca
      301 cactgcggag gcaggaggcg ctcccgggca ctgcccccgg ggagcccttg atctgccagg
      361 cgcagtgtct cagccagggc ccagatgtag ttgtaggcga agcgcagcgt ctcaatcttg
      421 gtgagcttgg tgtcgtcggg gaacgagggc agcacgctgc gcaaggcgtc cagcgcagcg
      481 ttgaggttgt gcatgcggtt gcgctcgcga tcgttggctt tgacgcgacg actcctccgc
      541 agggagtgca gcagagcctc ggaccgcacc cgagcgcgac ctcgccgcct ccgccgttcc
      601 tgctcttcgt cctgggcacc gggaacattc gatgccccgg agagggcggg agcgctcctc
      661 cgggctggca cggacagccc cgaggtggag gctaggggct gtagcctggc acagtcctcc
      721 tcgtcggtga ggaagctgga caggtcgctg ctgctgttgc tgctggagca gtcgagatca
      781 gagatgcagg tctccaaagg ggcaggcata ggcc
//
