LOCUS       sequence-103642-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving human Ngn1;
            constitutive expression of Blasticidine selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_hNgn1_UBC_Bla
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..27
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    107..125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    214..232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        280..318
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     315..339
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    358..382
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgatatcaac tttgtataga aaagttggct ccgaattcgc ccttcaggtc cgaggttcta
       61 gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat
      121 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      181 cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt
      241 cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag
      301 gcctatataa gcagagctcg tttagtgaac cgtcagatcg caaagggcga attcgaccca
      361 agtttgtaca aaaaagcagg ctgacctgcc accatgccag cccgccttga gacctgcatc
      421 tccgacctcg actgcgccag cagcagcggc agtgacctat ccggcttcct caccgacgag
      481 gaagactgtg ccagactcca acaggcagcc tccgcttcgg ggccgcccgc gccggcccgc
      541 aggggcgcgc ccaatatctc ccgggcgtct gaggttccag gggcacagga cgacgagcag
      601 gagaggcggc ggcgccgcgg ccggacgcgg gtccgctccg aggcgctgct gcactcgctg
      661 cgcaggagcc ggcgcgtcaa ggccaacgat cgcgagcgca accgcatgca caacttgaac
      721 gcggccctgg acgcactgcg cagcgtgctg ccctcgttcc ccgacgacac caagctcacc
      781 aaaatcgaga cgctgcgctt cgcttacaac tacatctggg ctctggccga gacactgcgc
      841 ctggcggatc aagggctgcc cggaggcggt gcccgggagc gcctcctgcc gccgcagtgc
      901 gtcc
//
