LOCUS       sequence-103644-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40 tdTomato L3-L2
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     107..123
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     108..124
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(231..254)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(429..448)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ctgggtatct agaggctcga ggtcgacggt
      121 atcgataagc ttgatatcga attcttactt gtacagctcg tccatgccgt acaggaacag
      181 gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
      241 ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc agttgcacgg gcttcttggc
      301 catgtagatg gtcttgaact ccaccaggta gcggccgccg tccttcagct tcagggcctg
      361 gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca
      421 gcccatggtc ttcttctgca ttacggggcc gtcggggggg aagttggtgc cgcgcatctt
      481 caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc
      541 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttctt
      601 gtaatcgggg atgtcggc
//
