LOCUS       sequence-103649-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving human Ngn1;
            constitutive expression of Blasticidine selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_hNgn1_UBC_Bla
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(76..474)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(654..673)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattgcgg taccgagctc
       61 gaattgtgct tagccttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc
      121 aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc
      181 gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat
      241 cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag
      301 ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc
      361 gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt
      421 agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatggtggc
      481 atctagagtc gacctgcagc ccaagcttcg tctaacaaaa aagccaaaaa cggccagaat
      541 ttagcggaca atttactagt ctaacactga aaattacata ttgacccaaa tgattacatt
      601 tcaaaaggtg cctaaaaaac ttcacaaaac acactcgcca accccgagcg catagttcaa
      661 aaccggagct tcagctactt aagaagatag gtacataaaa ccgaccaaag aaactgacgc
      721 ctcacttatc cctcccctca ccagaggtcc ggcgcctgtc gattcaggag agcctaccct
      781 aggcc
//
