LOCUS       sequence-103653-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human Rab7A fluorescently tagged with mCh on the N-terminus for 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Rowland AA, Chitwood PJ, Phillips MJ, Voeltz GK
  TITLE     ER Contact Sites Define the Position and Timing of Endosome Fission.
  JOURNAL   Cell. 2014 Nov 20;159(5):1027-41. doi: 10.1016/j.cell.2014.10.023.
  PUBMED    25416943
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.10"; journalName: "Cell"; date: "2014-11-20- 
            20"; volume: "159"; issue: "5"; pages: "1027-41"
COMMENT     Sequence Label: mCh-Rab7A
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..752
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(190..208)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     458..477
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      121 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      181 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      241 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      301 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      361 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      421 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      481 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      541 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      601 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      661 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      721 actccaccgg cggcatggac gagctgtaca agtccggact cagatctcga gctatgacct
      781 ctaggaagaa agtgttgctg aaggttatca tcctgggaga ttctggagtc gggaagacat
      841 cactcatgaa ccagtatgtg aataagaaat tcagcaatca gtac
//
