LOCUS       sequence-103655-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; constitutive expression of rtTA3 
            (TetON system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_hEF1a_rtTA3
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(81..788)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(798..822)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attgccggaa ttcaccactt
       61 tgtacaagaa agctgggtat ttacccgggg agcatgtcaa ggtcaaaatc gtcaagggca
      121 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc gccgctttcg
      181 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt
      241 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg
      301 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag
      361 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct
      421 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg
      481 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gtatgacttg
      541 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga
      601 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc
      661 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct
      721 tcgataccga caccattgag taattccaga gctccgttta tgactttgct cttgtccagc
      781 ctagacatgg tggcatcagc ctgctttttt gtacaaactt gggtcgaatt cgcccttggg
      841 atccacctga a
//
