LOCUS       sequence-10366-e        7038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Romm E, Nielsen JA, Kim JG, Hudson LD
  TITLE     Myt1 family recruits histone deacetylase to regulate neural 
            transcription.
  JOURNAL   J Neurochem. 2005 Jun . 93(6):1444-53.
  PUBMED    15935060
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurochem. 2005 Jun . 93(6):1444-53."
COMMENT     Sequence Label: pMyt1(502-736)
FEATURES             Location/Qualifiers
     source          1..7038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1116..1556
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1601..1612
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    2353..2474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2364..2383)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2418..2437
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2660..3115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2747..2766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2957..2978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3187..3544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3187..3378
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3210..3230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3395..3530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3886..4821
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    4879..4927
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4976..4995)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5095..5117
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5155..5173)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5241..5345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5346..5414,5415..6206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5564..5583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6377..6965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6866..6885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac
     1141 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
     1201 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
     1261 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
     1321 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
     1381 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
     1441 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
     1501 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg
     1561 aattcccggg gatccttaag cagcttgagg ttccacctta tgggagctac agacccaatg
     1621 tggctcccgc cacgcccagg gccaacttgg ccaaggagct ggagaagttc tccaaagtca
     1681 cctttgacta cgcaagtttt gatgctcagg tttttggcaa acgtatgctt gccccaaaga
     1741 ttcagaccag cgaaacctca cccaaagcct tccaatgctt cgattactct catgatgctg
     1801 aggctgcaca catggctgcc acagccatcc tgaacctctc cactcgatgt tgggagatgc
     1861 cagagaacct cagcacaaag ccacaggacc tccccagcaa ggctgtggac attgaggtag
     1921 atgagaatgg aactctggac ttgagcatgc ataaacatcg caagcgggaa aacactttcc
     1981 ccagcagtag cagctgcagt agcagccctg gtgtcaagtc tcctggtgtc tctcagcgtc
     2041 agagcagtac cagtgccccc agcagctcta tgacctcacc ccagtccagc caggcctctc
     2101 gccaggatga gtgggaccgt cctctagact acaccaaacc tagtcggctt cgagaggaag
     2161 agcctgagga gtcagagcca gcagcacact cttttgcttc ttctgaagca gatgatcagg
     2221 aggtgtcaga agagaacttt gaggagcgga agtatccagg ggaagtcacc ctaaccaact
     2281 ttgcggccgc aggtacctga ataactaagg ccgcttccct ttagtgaggg ttaatgcttc
     2341 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2401 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2461 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     2521 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat
     2581 cgatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2641 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2761 ttcccttcct ttcacgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3061 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     3121 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatcttccg
     3181 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3361 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3541 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagaat tctggctaca
     3601 ggtaagcgcc cctaaaatcc ctttgggcac aatgtgtcct gaggggagag gcagcgacct
     3661 gtagatggga cgggggcact aaccctcagg tttggggctt ctgaatgtga gtatcgccat
     3721 gtaagcccag tatttggcca atctcagaaa gctcctggtc cctggaggga tggagagaga
     3781 aaaacaaaca gctcctggag cagggagagt gctggcctct tgctctccgg ctccctctgt
     3841 tgccctctgg tttctcccca ggctcccgga cgtcctctag ccaccatgac ttcgaaagtt
     3901 tatgatccag aacaaaggaa acggatgata actggtccgc agtggtgggc cagatgtaaa
     3961 caaatgaatg ttcttgattc atttattaat tattatgatt cagaaaaaca tgcagaaaat
     4021 gctgttattt ttttacatgg taacgcggcc tcttcttatt tatggcgaca tgttgtgcca
     4081 catattgagc cagtagcgcg gtgtattata ccagacctta ttggtatggg caaatcaggc
     4141 aaatctggta atggttctta taggttactt gatcattaca aatatcttac tgcatggttt
     4201 gaacttctta atttaccaaa gaagatcatt tttgtcggcc atgattgggg tgcttgtttg
     4261 gcatttcatt atagctatga gcatcaagat aagatcaaag caatagttca cgctgaaagt
     4321 gtagtagatg tgattgaatc atgggatgaa tggcctgata ttgaagaaga tattgcgttg
     4381 atcaaatctg aagaaggaga aaaaatggtt ttggagaata acttcttcgt ggaaaccatg
     4441 ttgccatcaa aaatcatgag aaagttagaa ccagaagaat ttgcagcata tcttgaacca
     4501 ttcaaagaga aaggtgaagt tcgtcgtcca acattatcat ggcctcgtga aatcccgtta
     4561 gtaaaaggtg gtaaacctga cgttgtacaa attgttagga attataatgc ttatctacgt
     4621 gcaagtgatg atttaccaaa aatgtttatt gaatcggacc caggattctt ttccaatgct
     4681 attgttgaag gtgccaagaa gtttcctaat actgaatttg tcaaagtaaa aggtcttcat
     4741 ttttcgcaag aagatgcacc tgatgaaatg ggaaaatata tcaaatcgtt cgttgagcga
     4801 gttctcaaaa atgaacaata attctagccc tgaataagtg ataataagcg gatgaatggc
     4861 agaaattcgt cgaagcgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4921 ttgtgtgaat cgatagcgat aaggatcgga agatccgcgt atggtgcact ctcagtacaa
     4981 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5161 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5581 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7021 acatggctcg acagatct
//
