LOCUS       sequence-103670-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing CMV 
            promoter and human codon optimized Cas9 module Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV-hCas9 R4-R3
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..151)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(175..195)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcactaatac catctaagta gttgattcat agtgactgga tatgttgtgt tttacagtat
       61 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
      121 gtttctcgtt caactttatt atacaaagtt gtcctctaga gtcgcggccg ctcacacctt
      181 cctcttcttc ttggggtcag ccctgctgtc tccaccgagc tgagagaggt cgattcttgt
      241 ttcatagagc cccgtaattg actgatgaat cagtgtggcg tccaggacct cctttgtaga
      301 ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg aaggctgcag gcgcgcccaa
      361 gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc tccctgatgg gcttatccct
      421 gtgcttattg taagcagaaa gcaccttatc gaggttagcg tcggcgagga tcactctttt
      481 ggagaattcg cttatttgct cgatgatctc atcaaggtag tgtttgtgtt gttccacgaa
      541 cagctgcttc tgctcattat cttcgggaga ccctttgagc ttttcatagt ggctggccag
      601 atacaagaaa ttaacgtatt tagagggcag tgccagctcg ttacctttct gcagctcgcc
      661 cgcactagcg agcattcg
//
