LOCUS       sequence-103672-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression vector of 53BP1 28A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             211..234
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    235..259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ccaccatgga ctacaaagac
      181 catgacggtg atacatatga tcatgacatc gattacaagg atgacgatga caagacaagt
      241 ttgtacaaaa aagcaggctt catggaccct actggagctc agttggattc agatttcgct
      301 cagcaagata ctccttgcct gataattgaa gatgctcagc ctgaagcgca ggttctagag
      361 gatgattctg gttctcactt cagtatgcta tctcgacacc ttcctaatct ccagacgcac
      421 aaagaaaatc ctgtgttgga tgttgtgtcc aatcctgaac aaacagctgg agaagaacga
      481 ggagacggta atagtgggtt caatgaacat ttgaaagaaa acaaggttgc agaccctgtg
      541 gattcttcta acttggacac atgtggttcc atcgctcagg tcattgagca gttacctcag
      601 ccaaacagga caagcagtgt tctgggaatg tcagtggaat ctgctcctgc tgtggaggaa
      661 gagaagggag aagagttgga acagaaggag aaagagaagg aagaagatac ttcaggcaat
      721 actacacatt cccttggtgc tgaagatact gcctcagcgc agttgggttt tggggttctg
      781 gaactcgccc aggctcagga tgttgaggaa aatactgtgc catatgaagt ggacaaagag
      841 cagctacaat cagtaaccac caactctggt tataccaggc tgtctgatgt ggatgctaat
      901 actgcaatta agcatgaaga acagtccaac gaa
//
