LOCUS       sequence-103675-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (TAD residues 1-90) (72R) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-p53 (1-90) (72R)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(388..410)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(555..573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        641..745
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctggaagtt ctgttccagg ggcccctggg atccatggaa gaaccgcaga gcgatccgag
       61 cgttgaaccg cctctgagcc aagaaacctt tagcgatctg tggaaactgc tgccggaaaa
      121 taatgttctg agtccgctgc cgagccaggc aatggatgat ctgatgctga gtccggatga
      181 tattgaacag tggtttaccg aagatccggg tccggatgaa gcaccgcgta tgccggaagc
      241 agcaccgcgt gttgcaccgg caccggcagc acctacacca gcagctccag ctccggcacc
      301 gagctaagaa ttcccgggtc gactcgagcg gccgcatcgt gactgactga cgatctgcct
      361 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
      421 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      481 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc
      541 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      601 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      661 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      721 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      781 cttttttgcg gcatttt
//
