LOCUS       sequence-103678-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (TAD residues 1-90) (72R) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-p53 (1-90) (72R)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(346..369)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(396..418)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaacgcgcga aggcagatcg tcagtcagtc acgatgcggc cgctcgagtc gacccgggaa
       61 ttcttagctc ggtgccggag ctggagctgc tggtgtaggt gctgccggtg ccggtgcaac
      121 acgcggtgct gcttccggca tacgcggtgc ttcatccgga cccggatctt cggtaaacca
      181 ctgttcaata tcatccggac tcagcatcag atcatccatt gcctggctcg gcagcggact
      241 cagaacatta ttttccggca gcagtttcca cagatcgcta aaggtttctt ggctcagagg
      301 cggttcaacg ctcggatcgc tctgcggttc ttccatggat cccaggggcc cctggaacag
      361 aacttccaga tccgattttg gaggatggtc gccaccacca aacgtggctt gccagccctg
      421 caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg ggatagcttc
      481 aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg ggtccatgta
      541 taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat gatcaccatt
      601 taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag gtagcttgct
      661 aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg aaacaccgta
      721 tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt ttggacaacc
      781 acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact gtgttaattt
      841 aacatcacca tcaatataat aaggaaga
//
