LOCUS       sequence-103682-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express N-terminally FLAG tagged human USP13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Liu Y, Soetandyo N, Lee JG, Liu L, Xu Y, Clemons WM Jr, Ye Y
  TITLE     USP13 antagonizes gp78 to maintain functionality of a chaperone in 
            ER-associated degradation.
  JOURNAL   Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
  PUBMED    24424410
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014"; volume: "3:e01369. doi"; pages: " 10.7554/eLife.01369.
            Epub 2014 Jan 14"
COMMENT     Sequence Label: pRK5-FLAG-USP13
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             15..38
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcttgccgc catggactac aaggacgacg atgacaaggg gtcgacaatg cagcgccggg
       61 gcgccctgtt cggcatgccg ggcggcagcg gaggcaggaa gatggctgca ggagacatcg
      121 gcgagctgct agtgccccac atgcccacga tccgcgtgcc caggtccggc gacagggtct
      181 acaagaacga gtgcgccttc tcctacgact ctcccaattc tgaaggtgga ctctatgtat
      241 gcatgaatac atttttggcc tttggaaggg aacatgttga aagacatttt cgaaaaactg
      301 gacagagtgt atacatgcac ctgaaaagac atgtgcgaga gaaggtaaga ggggcgtctg
      361 gtggagcgtt accaaaaagg aggaattcca agattttttt agatctagat actgatgacg
      421 atttaaatag cgacgattat gaatatgaag atgaagccaa acttgttata ttcccagatc
      481 actatgaaat agcactacca aatattgagg agttaccagc cctggtaaca attgcttgtg
      541 atgcagttct cagctcaaaa tctccataca gaaagcagga cccagacacg tgggaaaatg
      601 aattgccagt atctaaatat gccaacaacc tcacccagct ggacaatgga gtcaggattc
      661 ctccaagtgg ttggaagtgt gccagatgcg acctgcgaga aaacctctgg ttgaatctga
      721 ctgacggctc tgtcctgtgt ggaaagtggt tctttgacag ctctgggggc aacgggcatg
      781 cgctggagca ttacagagac atgggctacc cactagccgt gaaactggga accatcactc
      841 ctgacgg
//
